Protease enzyme for ethanol Sunson PRN50
PRODUCT DESCRIPTION
The neutral protease is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. The product has been widely used in alcohol fermentation to degrade proteins in raw materials.
MECHANISM
The Neutral Protease catalyzes the hydrolysis of protein to produce free amino acids and short peptides within a pH range from weak acid to weak alkaline environment. Besides the main activity of endo-peptidase, the product also contains exo-peptidase, whose joint actions leads to liberation of short peptides and free amino acids.
REACTION PARAMETERS
PARAMETERS | RANGE |
Activity Temperature | 30℃-55℃ |
Optimum Temperature | 40℃-50℃ |
Activity pH | 5.5-8.5 |
Optimum pH | 6.5-7.5 |
PRODUCT SPECIFICATION
Items | Description |
Declared Activity* | 50,000u/g |
Physical Form | Powder |
Color** | Light brown |
Odour | Normal microbial fermentation odour. |
*Definition of Unit: 1 unit of Neutral Protease equals to the amount of enzyme, which hydrolyzes casein to get 1μg of tyrosine at 30℃ and pH7.5 in 1 min.
** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.
PRODUCT STANDARD
The product complies with GB1886.174.
No. | ITEMS | INDEX |
1 | Particle size (%<40 mesh) | ≥80 |
2 | Loss on drying/(%) | ≤8.0 |
3 | Lead/(mg/kg) | ≤5.0 |
4 | Arsenic/(mg/kg) | ≤3.0 |
5 | Total viable count/(CFU/g) | ≤50000 |
6 | Coliform Bacteria/(CFU/g) | ≤30 |
7 | Escherichia coli | (CFU/g) | <10 |
(MPN/g) | ≤3.0 |
8 | Salmonella/(25g) | Not Detected |
APPLICATION RECOMMENDATION
The recommended dosage is 0.05-3kg of the enzyme preparation per ton of total raw materials. The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.
SAFE HANDLING PRECAUTIONS
Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
WARNINGS
Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.
PACKAGE AND STORAGE
Ø Package： 25kgs/drum; 1,125kgs/drum.
Ø Storage： Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.
Ø Shelf life: 12 months in a dry and cool place.