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  • Release time: 2020 - 09 - 21
    $0
    PRODUCT DESCRIPTION This product is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is widely used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc.MECHANISMPullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharificat...
  • Release time: 2018 - 05 - 09
    $45
    Product CharacteristicsDeclared Activity2,000 u/mlProduction OrganismBacillus subtilisPhysical FormLiquidColorBrownColor may vary from batch to batch. Color intensity is not an indication of enzyme activity.OdourNormal microbial fermentation odourProduction MethodProduced by submerged fermentation, separation and extraction technology.Definition of Unit1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50℃. Product SpecificationLower LimitUpper LimitEnzyme activity2,000 u/mlLead...
  • Release time: 2018 - 05 - 09
    $45
    High-Temperature α-Amylase for starch Amer XL Product Characteristics Declared Activity70,000 u/mlProduction OrganismBacillus licheniformisPhysical FormLiquidColorBrown Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.OdourNormal microbial fermentation odourProduction MethodProduced by submerged fermentation, separation and formulation.Definition of Unit1 unit of High-Temperature α-Amylase equals to the amount of enzyme which liquefies 1mg of soluble starch to dextrin at 70℃ and pH6.0 in 1 min. Product SpecificationLower LimitUpper...
  • Release time: 2018 - 05 - 03
    $45
    Conzyme SPA-20 is a liquid form alkaline pectinase preparation. It is specially developed for bioscouring process of knitted natural cellulosic fibers such as cotton, linen, hemp and blend. It removes pectin and other impurities from cotton fibers without any degradation of the cellulose, soften and eliminate cotton seed to optimize dyeing performance. SpecificationAppearanceLiquidColorBrownOdorSlight fermentation odorSolubilitySoluble in waterCAS NO.9032-75-1  BenefitConzyme  SPA-20 is a perfect substitution to the traditional process of dyeing and finishing. It ...

PRODUCT DESCRIPTION

This product is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It is a thermal stable debranching enzyme able to work at low pH, and is widely used in brewing, starch sugar processing, monosodium glutamate and alcohol fermentation, etc.

MECHANISM

Pullulanase is a type of isoamylase which can selectively hydrolyze α-1,6-glucosidic linkage in pullulan, starch and oligosaccharides, thereby, making the complete hydrolysis of branched starch possible. Together with other enzymes (glucoamylase, β-Amylase) it speeds up saccharification and increase glucose or maltose yield. 

REACTION PARAMETERS  

PARAMETERS

RANGE

Activity Temperature

20℃-60℃

Optimum Temperature

45℃-52℃

Activity pH

3.0-6.8

Optimum pH

3.0-5.0

 PRODUCT SPECIFICATION

Items

Description

Declared Activity*

2,000 u/ml

Physical Form

Liquid

Color**

Light brown

Odour

Normal microbial fermentation odour.

*Definition of Unit: 1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50.

** Color: Color can vary from batch to batch. Color intensity is not an indication of enzyme activity.

PRODUCT STANDARD

The product complies with GB1886.174.

No.

ITEMS

INDEX

1

Bulk density/(g/ml)

≤1.20

2

pH/(25℃)

3.0-6.0

3

Lead/(mg/kg)

≤5.0

4

Arsenic/(mg/kg)

≤3.0

5

Total viable count/(CFU/mL)

≤50000

6

Coliform Bacteria/(CFU/mL)

≤30

7

Escherichia coli

(CFU/mL)

<10

(MPN/mL)

≤3.0

8

Salmonella/(25mL)

Not Detected

APPLICATION RECOMMENDATION

l  Glucose production: The recommended dosage is 0.35-0.6L per ton of dry starch together with glucoamylase together in saccharification step.

l  Maltose syrup: The recommended dosage is 1-2L per ton of dry starch together with Fungal Alpha-Amylase can together use in saccharification step.

l  In brewhouse: The recommended dosage is 0.4-0.8L of the enzyme preparation per ton of total raw materials. It’s used for degradation of amylopectin and production of dry beer. 

The dosage has to be optimized based on each application, the raw material specifications, product expectation and processing parameters. It is better to begin the test with the convenient volume.

SAFE HANDLING PRECAUTIONS

Enzyme preparations are proteins that may induce sensitization and cause allergic type of symptoms in susceptible individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa. Any direct contact with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.

WARNINGS

Keep sealed after use every time to avoid microbial infections and inactivation of enzymes until its finish.

PACKAGE AND STORAGE

Ø  Package25kgs/drum; 1,125kgs/drum.

Ø  Storage Keep sealed in a dry and cool place and avoid direct sunlight. Slight sedimentation is acceptable since it will not impact performance of the product.

Ø  Shelf life:  12 months in a dry and cool place.


For more details or any inquiries you may contact directly by sending us emails at: jeff2020@dingtalk.com



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